Atlético Nacional | Atlético Madrid | Club Atlético River Plate | Club Atlético Independiente | Club Atlético Lanús | Club Atlético Atlanta | Club Atlético 3 de Febrero | Club Atlético Huracán | Club Atlético Vélez Sársfield | Club Atlético River Plate (Uruguay) | Club Atlético Tigre | Atlético Huila | Nacional Atlético Clube Sociedade Civil Ltda. | Club Atletico River Plate Puerto Rico | Centro Atlético Fénix | Atlético Bucaramanga | Unión Atlético Maracaibo | Club Atlético San Isidro | Club Atlético Platense | Bangu Atlético Clube | Instituto Atlético Central Córdoba | Grêmio Atlético Farroupilha | Clube Atlético Paranaense | Clube Atlético Juventus | Club Deportivo Atlético Español | Club Atlético Vélez Sarsfield | Club Atlético River Plate Puerto Rico | Club Atletico River Plate | Club Atlético Banfield | Atlético Venezuela |
Atlético Minero and Sport Boys finished the 2008 season in 13th and 14th place, respectively, in the aggregate table and thus were relegated to the Segunda División.