He was the son of Sir Humphrey Mackworth of Gnoll, Glamorganshire, MP for Cardiganshire and was educated at Westminster School, Magdalen College, Oxford and the Inner Temple.
This led Mackworth to begin smelting copper at Neath, but found himself in direct competition with Sir Edward Mansel, who owned coal mining in the neighbouring areas.
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Herbert's son, Sir Humphrey's grandson, was Sir Herbert Mackworth who held the same Cardiff seat after his father until 1790.
Mackworth was the son of Humphrey Mackworth of Betton Grange.
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