Infante Edward, 5th Duke of Guimarães, son of Infante Edward, 4th Duke of Guimarães and his wife, Isabella of Braganza, never married
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Infante Edward, 4th Duke of Guimarães, son of King Manuel I of Portugal and Maria of Aragon, married Isabella of Braganza
Yet, King Edward began to contest these grand privileges during his reign, and King John III of Portugal finally stripped them of those rights in 1537, investing his brother Edward, with the seigneurial titles.