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Lok married firstly, about 1562, Jane Wilkinson, the daughter of William Wilkinson (d.1543), mercer and Sheriff of London in 1538, by Joan North (d.1556), only sister of Edward North, 1st Baron North, and daughter of Roger North (d.1509) and Christian Warcop.
Her father was Sheriff in 1548, and was knighted in that year by the young Edward VI.