The song's title most likely refers to the line in the Pledge of Allegiance "One nation under God".
The Nation | Navajo Nation | Groove Armada | VNV Nation | Nation | Nation of Islam | nation | Confederated Salish and Kootenai Tribes of the Flathead Nation | The Birth of a Nation | Osage Nation | Crow Nation | Cherokee Nation | Alien Nation | Talk of the Nation | Choctaw Nation of Oklahoma | The Savage Nation | Oneida Indian Nation | Krush Groove | Hip-Hop Nation | The Groove Tube | Terry Nation | Roc Nation | Fast Food Nation | Eight-Nation Alliance | Acumen Nation | The Emperor's New Groove | Six Nations of the Grand River First Nation | Petite-Nation River | One Nation (Australia) | One Nation |
In some ways, Uncle Jam Wants You (a reference to the "Uncle Sam wants you!" US Army recruitment posters) is a more militant sequel to the group's previous album, One Nation Under a Groove.
The rest of the song is jams of various Funkadelic or Parliament songs, such as "Cosmic Slop" and "Cholly (Funk Getting Ready to Roll!)", as well as John Frusciante's "Untitled #2".