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His father, William Harcourt Isham Mackworth (1806—1872), a younger son of Sir Digby Mackworth, the 3rd Baronet, took the additional surname Dolben after he married Frances, the heiress of Sir John English Dolben, the 4th Baronet.
Sir Thomas Metcalfe, 4th Baronet (1795–1853), East India Company servant, agent to Governor General of India, son of the above