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His father, William Harcourt Isham Mackworth (1806—1872), a younger son of Sir Digby Mackworth, the 3rd Baronet, took the additional surname Dolben after he married Frances, the heiress of Sir John English Dolben, the 4th Baronet.
Baptised at St Anne's Church, Soho on 29 March 1739, he was the eldest surviving son of the Rt Revd Sir William Ashburnham, 4th Baronet, Bishop of Chichester and his wife Margaret Pelham, daughter of Thomas Pelham.
Sir William Ashburnham, 5th Baronet (1739–1823), his son, British MP for Hastings 1761–1774
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Sir William Ashburnham, 2nd Baronet (1678–1755), British MP for Hastings 1710–1713, 1722–1741, for Seaford 1715–1717