Niccolò experienced further tragedy in 1512 when the French invaded Brescia during the War of the League of Cambrai.
Treaty of Blois (1509) (3rd Treaty of Blois), of December 12, 1509, an alliance between Ferdinand II of Aragon (and now regent of Castile), Holy Roman Emperor Maximilian I, and King Louis XII of France in the War of the League of Cambrai in northern Italy.
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He returned to Belluno later, but fled to Rome in 1509 to escape the occupation of the city by the Holy Roman Empire during the War of the League of Cambrai.