Western blot analysis shows that homozygous cycle0 mutants have very little PER and TIM protein as well as low per and tim mRNA levels.
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Granzyme secretion can be detected and measured using Western Blot or ELISA techniques.
While performing molecular assays involving use of antibodies such as in the Western blot, immunohistochemistry, and ELISA, one should carefully choose antibodies that recognize linear or conformational epitopes.
This offering enables a single solution for managing clinical study data, treatment groups data, subject data, sample data and assay data from diverse assays such as Immunohistochemistry, Western Blot, PCR, etc.
A controversy erupted in National Centre for Cell Science (NCCS), Pune in 2006 when an anonymous mail alleged that the authors (H. Rangaswami and Colleagues from the group of Dr. Gopal Kundu) may have misrepresented data (especially through Western blots) in a paper published in Journal of Biological Chemistry.