Fountaingrove has significant aquatic as well as upland biota, and is noted for being a habitat of the Western pond turtle, Actinemys marmorata, a "Species of Special Concern".
Western pond turtles originally ranged from northern Baja California, Mexico, north to the Puget Sound region of Washington.
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The watershed is currently home for many rare, threatened and endangered species including fairy shrimp, bald and golden eagles, Alameda whipsnake, western pond turtle, California tiger salamander, California red-legged frog, San Joaquin kit fox, and the San Francisco dusky-footed wood rat.