454 Life Sciences, is a biotechnology company based in Branford, Connecticut.
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In May 2007, Project "Jim", a project initiated by Rothberg and 454 Life Sciences to determine the first sequence of an individual was completed after sequencing the genome of James Watson, co-discoverer of the structure of DNA.
The genome was sequenced by a hybrid approach using 454 Life Sciences and Illumina, achieving a total of 340X coverage, with 99.8% sequence identity between the two methods.
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Emulsion PCR is used in the methods developed by Marguilis et al. (commercialized by 454 Life Sciences), Shendure and Porreca et al. (also known as "Polony sequencing") and SOLiD sequencing, (developed by Agencourt, later Applied Biosystems, now Life Technologies).
In 1999, Rothberg founded 454 Life Sciences, based in Branford (CT), which pioneered an entirely new way to sequence genomes.