He worked with his father, Charles Frédéric Dubois (1804–1867), in the production of Les Oiseaux de l’Europe et leurs œufs, completing it after his father’s death.
He was the author of Planches colorées des oiseaux de l’Europe ("Color plates of the birds of Europe") and Catalogue systématique des Lépidoptères de la Belgique ("Systematic catalog of the Lepidoptera of Belgium"), which was completed by his son, Alphonse Joseph Charles Dubois (1839–1921), after his death.
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