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Three-year-old Dorothea and her sister and brother followed their exiled parents to Veere in Zeeland, the Netherlands, and were taken care of by the Dutch regents, their grandaunt and aunt, Margaret of Austria and Mary of Hungary.
The Berlin Virgin formed the other half of a diptych with the Washington portrait of Diego de Guevara, a Spanish courtier with the Habsburgs, otherwise best known for giving the Arnolfini Portrait to Archduchess Margaret of Austria, governor of the Spanish Netherlands.