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Nichols took part in the Lord Bandon dredging expedition of 1886 with Haddon, sponsored by the Royal Irish Academy, and in the biological surveys of Lambay, Clare Island and Malahide.
A keen amateur botanist, he later became interested in Mollusca and recorded the marine Mollusca collected during the Clare Island Survey.
She was a specialist in Porifera and Cnidaria with both Scharff and Stephens taking part in the Royal Irish Academy Clare Island Survey.