His work resulted in compounds which he used to treat patients at least one year before the commonly accepted discovery of penicillin by Alexander Fleming.
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Picado received the academic rank of Doctor in science in 1913, that same year he was admitted to the Pasteur Institute and the Colonial Institute of Paris.
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Although, the discovery of penicillin has been attributed to Alexander Fleming, Picados' old laboratory notebooks from 1923 show records of the antibiosis of penicillium sp.