It appears as comic relief in The Simpsons episodes "The Last Temptation of Homer" and "Midnight Rx"; as well as on Family Guy in the episode "E. Peterbus Unum" where Stewie is curious about the sound Achmed "makes when you're about to assassinate an infidel".
E pluribus unum | Unum | E PLURIBUS UNUM | E Pluribus Unum |