In the Blizzard computer game StarCraft, the Terran dropship unit quotes "In the pipe, five by five" when confirming a move order.
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In the episode Welcome To The Kree empire of the animated series The Avengers: Earth's Mightiest Heroes Ms Marvel goes in pursuit of an Kree spaceship.
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In the series Buffy the Vampire Slayer, Faith Lehane used the phrase "Five by Five" to describe something being good.
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In the video game Call of Duty: Modern Warfare 3, SEAL Delivery Vehicle Team 4 (SDV Team 4) uses it as a radio check respond to Sandman (Metal 0-1), "Roger, 0-1. We have you five-by-five. Phase line Echo secured. We have execute authority."
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In a parody of this scene, the South Park episode "Raising the Bar" James Cameron says "We're in the pipe, five by five" as he lowers himself deeper into the ocean to find the "bar" that represents shameful feelings.
Also see Five by five for the phrase "loud and clear" in communications.