A French expedition, organized by Protestant leader Admiral Gaspard de Coligny and led by the French Explorer Jean Ribault, had landed at the site on the May River (now the St. Johns River) in February 1562, before moving north to Port Royal Sound.
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French Huguenots founded Fort Caroline in modern-day Jacksonville in 1564, but this fort was conquered by forces from the new Spanish colony of St. Augustine the following year.