X-Nico

unusual facts about Gram staining


Gram staining

The method is named after its inventor, the Danish scientist Hans Christian Gram (1853–1938), who developed the technique while working with Carl Friedländer in the morgue of the city hospital in Berlin in 1884.


Alcian blue stain

Alcians blue carries Phthalocyanine one of the most highly colored chromophores yet known with a molar extinction of 120,000 i.e. AB is detectable at half the molar concentration of popular dyes like toluidine blue, tryarylmethanes (e.g. pararosaniline and the analogous Schiff bases used in PAS stain, Crystal violet in Gram stain) etc.


see also