In 1925 Lorry McKenzie commenced operating a service in Healesville.
The Yarra Valley Railway currently run a Walker railcar from Healesville to the Tarrawarra Winery, crossing the Watts River, under the Donovan's Road overbridge and through the tunnel.
The Internet Movie Database has Healesville Railway Station as a filming location for the Australian short film Harry's War (1999).
It is planned to link the Princes Freeway at Pakenham to the South Gippsland Highway at Koo Wee Rup using the existing alignment of the Healesville - Koo Wee Rup Road.
In March 1863 after three years of upheaval, the surviving leaders, among them Simon Wonga and William Barak, led forty Wurundjeri, Taungurong (Goulburn River) and Bun warrung people over the Black Spur and squatted on a traditional camping site on Badger Creek near Healesville and requested ownership of the site.