#Inoculate nitrate broth with an isolate and incubate for 48 hours.
Essendon Place was the seat of the Barons Dimsdale of Russia; Thomas Dimsdale was an expert on the treatment of smallpox by inoculation and in 1768 he was invited to Russia to inoculate Catherine the Great.
After Francis' death, Franklin became involved in promoting inoculation in Philadelphia: he published many studies on its value, working with several physicians, including the famed William Heberden at the Pennsylvania Hospital, which he helped found.
Rostraureum tropicale is pathogenic towards Terminalia ivorensis and a closely related host, Terminalia superba, causing well-developed stem cankers within six weeks of inoculation.
At this time, British health officials began to press for widespread vaccination using Waldemar Haffkine’s plague vaccine, although the government stressed that inoculation was not compulsory.
On 17 March 1791 he was elected physician to the smallpox and inoculation hospitals at St. Pancras, in succession to Edward Archer.