In Johnson County, the road intersects the southern terminus of KY 2381 before it passes to the northeast of Paintsville-Prestonsburg Combs Field, where it intersects KY 2559.
•
The route passes to the east of Prestonburg Community College and narrows to a two-lane unnamed road, curving northeast through woods and running to the east of Levisa Fork.
•
At this point, KY 321 turns east to form a concurrency with KY 3, passing through more rural areas with some development including Highlands Regional Medical Center.
Louisville, Kentucky | Kentucky | Lexington, Kentucky | University of Kentucky | Kentucky Derby | Versailles, Kentucky | U.S. Route 66 | U.S. Route 1 | Covington, Kentucky | Frankfort, Kentucky | Paducah, Kentucky | U.S. Route 6 | U.S. Route 101 | Route 66 | Bowling Green, Kentucky | U.S. Route 30 | New Jersey Route 4 | European route E65 | U.S. Route 40 | California State Route 1 | Barren County, Kentucky | U.S. Route 1 in Maine | Pennsylvania Route 309 | European route E18 | Ashland, Kentucky | U.S. Route 23 | Governor of Kentucky | European route E4 | U.S. Route 9 in New York | U.S. Route 11 |