Using a standard laboratory method to determine antimicrobial susceptibility, methanol-based extracts of Lycoperdon umbrinum fruit bodies were shown in a 2005 study to have "significant" antibacterial activity against various human pathogenic bacteria, including Bacillus subtilis, Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, Streptococcus pyogenes, and Mycobacterium smegmatis.
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According to Curtis Gates Lloyd, American specimens have more slender spines than European ones.