This activity facilitates phosphorylation of important downstream kinetochore targets (such as tension-sensing 3F3/2 phospho-epitope) of Plk1, which is important for recruitment of spindle assembly checkpoint proteins such as Mad1 and proper kinetochore-microtubule attachment.
BUB1 is the other kinase that recruits Mad1 to kinetochores and activates it if a kinetochore is unattached.
Mad1 |