The recognition sites for HindIII, SphI, PstI, SalI, XbaI, BamHI, SmaI, KpnI, SacI and EcoRI restriction enzymes have been derived from the vector M13mp19.
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The cells which have taken up the plasmid can be differentiated from cells which have not taken up the plasmid by growing it on media with Ampicillin.