Roux got his baccalaureate in sciences in 1871 and started his studies in 1872 at the Medical School of Clermont-Ferrand.
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He studied its toxin and its properties, and began in 1891 to develop an effective serum to treat the disease, following the demonstration, by Emil Adolf von Behring (1854–1917) and Kitasato Shibasaburō (1852–1931) that antibodies against the diphtheric toxin could be produced in animals.
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