Also, it is a very flexible technique that enables variable application including BAC (bacterial artificial chromosome) and bacterial genome resequencing, as well as SAGE (serial analysis of gene expression) tag and barcode sequencing.
•
Streptavidin has a very strong affinity for biotin, thus the forward primer will bind firmly on the surface of the beads.
DNA sequencing | Natacha Polony | Polony sequencing | Compression of Genomic Re-Sequencing Data |
Emulsion PCR is used in the methods developed by Marguilis et al. (commercialized by 454 Life Sciences), Shendure and Porreca et al. (also known as "Polony sequencing") and SOLiD sequencing, (developed by Agencourt, later Applied Biosystems, now Life Technologies).