Activation of various secreted factors (Vg1, Nodal, Wnt8C, FGF8 and Chordin) and transcription factors (Brachyury and Goosecoid) adjacent to the site of streak formation is required for this process.
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To form the streak, reptiles, birds and mammals arrange mesenchymal cells along the prospective midline, establishing the second embryonic axis, as well as the place where cells will ingress and migrate during the process of gastrulation and germ layer formation.
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FGF8 is implicated in the process of this dispersal from the primitive streak.