In the second episode of the first series she insisted her husband wear a tie to prune the garden.
When lipid diacylglycerol (DAG) binds to the C1 domain, CHN1 is transferred to the plasma membrane and negatively regulates Rho-family small GTPases RAC1 and CDC42, thus causing the morphological change of axons by pruning the ends of axon dendrites.
A study on orchard mango trees in Nelspruit, South Africa, compared open vase, closed vase, central leader, palmette and standard pruning systems and recommended a modified pyramid, somewhere between a central leader and a closed vase system, for high-density mango orchards.
Another heuristic for dealing with the zugzwang problem is Omid David and Nathan Netanyahu's verified null-move pruning.