Species of Pseudotocinclus are found in the upper Tietê River basin in the ribeirão Grande, a tributary of the middle course of the rio Paraíba do Sul, Pindamonhangaba, in the headwaters of rio Itatinga, a coastal river of São Paulo, and in the upper and lower courses of the Juquiá River of the Ribeira de Iguape River basin, all rivers of São Paulo, Brazil.