Following the refusal, Osborne filed a claim for due process under 42 U.S.C. §1983, challenging a State's "deprivation of any rights... secured by the Constitution" and requested the DNA evidence against him be tested at his personal expense by Short Tandem Repeat (STR) analysis, a method more discriminating than both RFLP and DQ Alpha, and unavailable at the time of his trial.
DNA-based and/or molecular- A unique gene (DNA sequence), occurring in proximity to the gene or locus of interest, can be identified by a range of molecular techniques such as RFLP, RAPD, AFLP, DAF, SCAR, microsatellite, or single-nucleotide polymorphism (SNP) detection.
Researchers at the University of Nottingham demonstrated that the DNA extracted from both green and roasted beans could be used in a restriction fragment length polymorphism (RFLP) based analysis to differentiate between Arabica and Robusta types of coffee.