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His father, William Harcourt Isham Mackworth (1806—1872), a younger son of Sir Digby Mackworth, the 3rd Baronet, took the additional surname Dolben after he married Frances, the heiress of Sir John English Dolben, the 4th Baronet.
He was the second son of Thomas Barrington, in turn first son of Sir John Barrington, 3rd Baronet, and his wife Lady Anne Rich, daughter of Robert Rich, 3rd Earl of Warwick.