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His father, William Harcourt Isham Mackworth (1806—1872), a younger son of Sir Digby Mackworth, the 3rd Baronet, took the additional surname Dolben after he married Frances, the heiress of Sir John English Dolben, the 4th Baronet.
In 1770, he married Anne Miller, daughter of Sir John Miller, 4th Baronet by whom he had a son, William Charles (1772–1849).