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His father, William Harcourt Isham Mackworth (1806—1872), a younger son of Sir Digby Mackworth, the 3rd Baronet, took the additional surname Dolben after he married Frances, the heiress of Sir John English Dolben, the 4th Baronet.
Throughout his life he corresponded with continental scholars, by whom he was much esteemed, as evidenced by Gronovius's dedication to Ellys of his edition of Ælian's Varia Historia, and the Wetsteins' edition of Johann Caspar Suicer's Thesaurus Ecclesiasticus, to which he had contributed the use of a manuscript.