The main units that were stationed there were from No. 6 Group RCAF, including No 77 Sqn; No 420 Sqn; No 425 Sqn No 431 Sqn and No 434 Sqn.
From August 1940 to December 1940, Tholthorpe was a landing field for Whitley bombers of No. 58 Squadron RAF and No. 51 Squadron RAF based at Linton.