The Throckmorton family were infamous in England for their part in the Throckmorton Plot of 1583 which aimed to murder Elizabeth I and replace her with Mary Stuart.
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In 1584 Gentili and Jean Hotman (1552—1636) were asked by the government to advise on the treatment of Spanish ambassador Bernardino de Mendoza (about 1540–1604), who had been implicated in the so-called Throckmorton plot against Queen Elizabeth I.