A test tube is prepared with basophile stimulation buffer (BSB) including Interleukin 3 and an allergen which is to be tested.
A randomized, double-blind, placebo-controlled cross-over study by the University Medical Center Groningen reported that dishydrosis outbreaks on the hands increased significantly among those allergic to house dust mites, following inhalation of house dust mite allergen.
Most had higher titres of antibodies against Fel d 4 than against Fel d 1, another prominent cat allergen.