Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin.
Chemical treatments- anionic detergent: (Sodium dodecyl sulfate (SDS)), enzymatic agent (Trypsin), non-ionic detergent (Triton X-100) are all agents used to remove cells from the ECM scaffold by disrupting cellular proteins, while not affecting the mechanical strength and functional structure of the ECM through the maintenance of the collagen and elastin.