Elisabeth and her older sister Archduchess Maria Annunziata of Austria (later Abbess of the Theresia Convent in the Hradschin, Prague) were the product of his third marriage to Infanta Maria Theresa of Portugal, a daughter of deposed King Miguel I of Portugal.
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Hans-Adam II, Prince of Liechtenstein is descended from Isabella I and Ferdinand II through his grandmother, Archduchess Elisabeth Amalie of Austria; Elisabeth Amalie descends from the Iberian couple via the Spanish and Hesse-Darmstadt houses, as well as through the formerly-reigning Catholic imperial or royal houses of Austria-Hungary, Portugal, and Bavaria (these formerly-reigning houses all descend from Isabella I and Ferdinand II).