Kozlov married Elizabeth V. Kozlova, a woman 29 years his junior, who accompanied him on his final journey of exploration as the expedition ornithologist, and who was to publish many monographs and scientific papers on the avifauna of Central Asia.
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She studied medicine at the New York Medical College and Hospital for Women, was licensed in 1899, and began the practice of medicine in 1900 in Schenectady.