After receiving his medical degree, Michaelis worked as a private research assistant to Moritz Litten (1899–1902) and for Ernst Viktor von Leyden (1902–1906).
In 1912 she moved to Berlin where she worked with Leonor Michaelis and co-authored their paper in Biochemische Zeitschrift (1913;49:333–369) which showed that the rate of an enzyme-catalyzed reaction is proportional to the amount of the enzyme-substrate complex.
In a seminal paper in 1913, Michaelis and Menten derived the equation in more detail and interpreted it more profoundly.
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Notably, his work was taken up by German biochemist Leonor Michaelis and Canadian physician Maud Menten.
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