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His father, William Harcourt Isham Mackworth (1806—1872), a younger son of Sir Digby Mackworth, the 3rd Baronet, took the additional surname Dolben after he married Frances, the heiress of Sir John English Dolben, the 4th Baronet.
The son and heir of Sir Francis Kinloch, 2nd Baronet, of Gilmerton, by his spouse Mary, daughter of David Leslie, 1st Lord Newark, he succeeded his father in 1699, and married circa 1705, Mary (d. 2 April 1749, Gilmerton House, East Lothian), daughter and co-heiress of Sir James Rocheid, Baronet, of Inverleith (d. after 1704).