Older cultures (>18 days in-vitro) receiving the same experimental conditions, increase mEPSC frequency and maintain the same mEPSC amplitude.
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A long-term, concurrent confocal microscopy and electrophysiology investigation conducted on cortical rat in-vitro neural networks (age > 3 weeks in-vitro) growing on Multi Electrode Arrays examined the correlation between network activity levels and changes in the sizes of individual synapses.
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The study demonstrated inducing 2 days of post-synaptic activity blockade (via pharmaceutical application of TTX) in younger cultures (≤2 weeks in-vitro) increased mEPSC amplitude and did not change mEPSC frequency.
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Intra-cellular recordings show robust increase in mEPSC amplitude (approximately 130% above control values) following 4–5 hours of TTX treatment.
Image scaling | Organ flue pipe scaling | Multidimensional scaling | image scaling | Dynamic voltage scaling | Dynamic frequency scaling |