For example, while the human genome contains an average of 8.4 introns/gene (139,418 in the genome), the unicellular fungus Encephalitozoon cuniculi contains only 0.0075 introns/gene (15 introns in the genome).
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Comparison of mtDNA cytochrome b and NADH dehydrogenase subunit 2 as well as nuclear β-fibrinogen intron 7 sequence data indicates that this is incorrect (Winker & Pruett, 2006).
The first intron of the CSFR1 gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene, oriented in the opposite direction to the CSFR1 gene.
The technique has largely been supplanted by the approach of sequencing cDNA generated from mRNA and then using bioinformatics tools such as NCBI's BLAST server to determine the source of the sequence, thereby identifying the appropriate exon-intron splice sites.
Sidney Altman and Thomas Cech first discovered catalytic RNA molecules, RNase P and group II intron ribozymes, in 1989 and won the Nobel Prize for their discovery.
mtDNA NADH dehydrogenase subunits 2 and 3 and nDNA β-fibrinogen intron 7 sequence data puts it closer to (but still well distant from) the Streaked Bulbul (Ixos malaccensis).
miR-214 is likely to have emerged within the Dnm3 gene after the divergence of jawed and jawless vertebrates and is located on the opposite strand of an intron of Dnm3 and is associated in an expression cluster with miR-199.
Recent cladistic analysis of mtDNA cytochrome b, cytochrome c oxidase subunit I and NADH dehydrogenase subunit 2, as well as nuclear β-fibrinogen intron 7 sequence data, combined with a distinct morphology and behaviour, suggest that the genus is valid but not monotypic.
There are 17 species of Patagioenas, which can be assigned to 4 groups based on mtDNA cytochrome b, cytochrome c oxidase subunit I, and NADH dehydrogenase subunit 2, as well as the nuclear β-fibrinogen intron 7 data combined with analyses of vocalizations and morphology.
PRPF31 is recruited to introns following the attachment of U4 and U6 RNAs and the 15.5K protein NHP2L1.
The TIMP3 gene is located within an intron of this gene and is transcribed in the opposite direction.
The TIMP4 gene is located within an intron of this gene and is transcribed in the opposite direction.
Complementary binding between U2 snRNA (in an area lying towards the 5' end but 3' to hairpin I) and the branchpoint sequence (BPS) of the intron results in the bulging out of an unpaired adenosine, on the BPS, which initiates a nucleophilic attack at the intronic 5' splice site, thus starting the first of two transesterification reactions that mediate splicing.
When testing the effect of unc-5::intron::unc-5 transgene on a mislocalization defect in UNC-5 mutant animals at 25°C a significant rescue of the mislocalization defect was observed.