She has made several appearances in other media, most notably in the cartoon, Captain N: The Game Master.
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Mother Brain makes multiple appearances in other media, most notably in the television show Captain N: The Game Master, where she serves as the primary antagonist to the protagonists.
Proto Man's first game appearance, Mega Man 3, was adapted for animation in the Captain N: The Game Master episode "A Tale of Two Dogs".
The game was featured in the episode "Once Upon a Time Machine" in the second season of the video game-themed cartoon series, Captain N: The Game Master.
In fact, the Level 1 background music was also used in later season 2 episodes of Captain N: The Game Master.
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The background music for Level 1 and Level 2 was used in the beginning of the Captain N: The Game Master episode, Trouble With Tetris, in a slightly altered form.
Bayou Billy also received some amount of exposure on the Nintendo-themed animated TV series, Captain N: The Game Master, where Bayou Billy (modeled somewhat like Crocodile Dundee) appears in an episode titled "How's Bayou".
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The comics are based upon Nintendo video game and television intellectual property from Super Mario Bros., Game Boy, The Legend of Zelda, Captain N: The Game Master, Metroid, and Punch-Out!!.