In the show, she was voiced by Levi Stubbs, Jr. of the Four Tops, who was also well known for playing the voice of Audrey II in the film Little Shop of Horrors.
•
She has made several appearances in other media, most notably in the cartoon, Captain N: The Game Master.
•
Mother Brain makes multiple appearances in other media, most notably in the television show Captain N: The Game Master, where she serves as the primary antagonist to the protagonists.
Mother Teresa | How I Met Your Mother | Queen Elizabeth The Queen Mother | brain | Mother's Day | Mary (mother of Jesus) | Mother Jones | Mother Goose | Mother Jones (magazine) | Mother Father Brother Sister | Pinky and the Brain | Mother India | brain tumor | Queen Mother | No Brain | Traumatic brain injury | Mother Nature | I Mother Earth | Cat Mother & the All Night Newsboys | Brain tumor | Billion Dollar Brain | traumatic brain injury | Mother Courage and Her Children | Mother Courage | Divine Mother | Bryan "Brain" Mantia | Brain Age: Train Your Brain in Minutes a Day! | Working Mother | Whistler's Mother | The Mother |
These are the first seven bosses: Mike Tyson; Mecha Birdo; Dracula; Kraidgief (a parody of palette-hacked character glitches); Mother Brain; Bowser, Wart and Dr. Wily in the Koopa Klown Kar; and a mix between the Mecha Dragon from Mega Man 2 and the Yellow Devil from Mega Man and Mega Man 3.
Simon Belmont and Mega Man were appropriately replaced by Samus Aran, the Metroid heroine who had not appeared in the cartoon, and Mother Brain's second-in-command became Uranos, the demigod from Kid Icarus.