The concept of enzyme replacement therapy was introduced in 1964 by Christian de Duve.
Christian de Duve, then chairman of the Laboratory of Physiological Chemistry at the Catholic University of Louvain in Belgium, had been studying the mechanism of action of a pancreatic hormone insulin in liver cells.
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After a postdoctoral fellowship at Rockefeller University with Christian de Duve, he became professor at the Catholic University of Santiago, in Chile.