X-Nico

3 unusual facts about Coomassie Brilliant Blue


Two-dimensional gel electrophoresis

These proteins can then be detected by a variety of means, but the most commonly used stains are silver and Coomassie Brilliant Blue staining.

To obtain a separation by size and not by net charge, as in IEF, an additional charge is transferred to the proteins by the use of Coomassie Brilliant Blue or lithium dodecyl sulfate.

Zymography

The zymogram is subsequently stained (commonly with Amido Black or Coomassie Brilliant Blue), and areas of digestion appear as clear bands against a darkly stained background where the substrate has been degraded by the enzyme.



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