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3 unusual facts about Fast protein liquid chromatography


Fast protein liquid chromatography

FPLC was developed and marketed in Sweden by Pharmacia in 1982 and was originally called fast performance liquid chromatography to contrast it with HPLC or high-performance liquid chromatography.

The range of purity required can be from that required for basic analysis (SDS-PAGE or ELISA, for example), with only bulk impurities removed, to pure enough for structural analysis (NMR or X-ray crystallography), approaching >99% target molecule.

FPLC

Fast protein liquid chromatography, a technique used to separate or purify proteins from complex mixtures



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