The 300 subseries entered service on the Fukuchiyama Line, while shortened consists began running on the Nara Line, Kosei Line, Kusatsu Line, and on the Sanyō Main Line in the Okayama area on the Sunliner service.
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A special JR West rapid service train (501D) from Kyoto bound for Shigaraki had entered the Shigaraki Line at Kibukawa Station from the Kusatsu Line, transporting 716 passengers to the World Ceramic Festival that was being held in Shigaraki at the time.